Showing 2 results for Bessalah
Salma Bessalah, Samira Jbahi, Mouldi Zagrouba, Hajji Sawsen, Amel Raoufi, Mustpha Hidouri,
Volume 19, Issue 2 (June-Biomaterials Special Issue- 2022)
Abstract
Abstract
In this research, Gelatine (GEL)/ Chitosan (CH) wound dressing was prepared and irradiated with gamma rays from 60Co source for wound healing applications. GEL-CH composite characterization and functional properties were determined. The structural changes occurring after γ-irradiation at doses from 5 to 25 kGy were reported by physico-chemical techniques such as Electron Paramagnetic Resonance (EPR), Fourier Transform Infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Electrochemical Impedance Spectroscopy (EIS) studies. The antioxidant capacity was studied using DPPH (1,1-diphenyl-2-picrylhydrazyl free radical) scavenging and the antibacterial activities of Staphylococcus aureus and Escherichia coli were observed using liquid medium. Results revealed that EPR spectroscopy of un-irradiated GEL-CH showed 2 paramagnetic centers correspond to g=2.077 and g= 2.079. After irradiation, no active centre was appeared. A dose-dependent decrease in the central signal intensity was detected, then the EPR signal intensity almost disappears at 20 kGy. Gamma rays caused a slight increase in ion conductivity. FTIR suggest a slightly crosslinking phenomenon at 20 kGy. The XRD analysis does not show peak indicating crystallinity between a range of 2θ (15–30°). Moreover, γ-irradiation elevated the Scavenging DPPH radical activity (0.75 ± 0.07%). Gamma rays did not affect the antibacterial activity of GEL-CH wound dressing against pathogenic bacteria. The innovative results showed that the required γ-radiation for sterilization was ranged from 5 to 25 kGy. It permits to improve the physico-chemical and biological properties and maintain the native structural integrity of the GEL/ COL wound dressing
Salma Bessalah, Jebahi Samira, Amel Raoufi, Asim Faraz, Mouldi Zagrouba, Mohamed Hammadi,
Volume 19, Issue 2 (June-Biomaterials Special Issue- 2022)
Abstract
Abstract
Gelatin (GEL) is most extensively used in various fields, particularly in therapeutics and pharmaceuticals. GEL was extracted from goat skin using hot temperature extraction process and compared with that of commercial GEL. The physico-chemical characterization and functional properties were investigated by using temperature denaturation (Td), water-holding and fat-binding capacities (WHC and FBC), colour measurement and UV-light spectrum. In vitro biocompatibility was studied for the first time and was evaluated by blood coagulation index (BCI) and haemolytic tests for using as wounds dressing. The results revealed thermal stability of goat GEL at Td 37°C. WHC and FBC capacities represented 2.5 and 1.2 g/ml, respectively. The hunter colour spaces a*, b* and L* showed a -0.27, -1.97 and 25.23 values, respectively. UV-Vis absorption spectrum of the goat GEL showed a maximum absorption peak at 280 nm. The in vitro anticoagulant activities of extracting GEL were higher than 70% after incubation for one hour. After being in contact with red blood cells for 1 h, the haemolysis ratio increased from to 0.46 to 1.4 when the concentration of goat GEL increased from 1 to 50 mg/ml suggesting the safety of the tested samples. These results suggest that thromboresistivity and hemocompatibility of this biopolymer retained the biological activity of our samples for biomaterial applications. According to this, goat GEL successfully competes with, and significantly could be useful for substitution of bovine in wound healing.
